杭州浙大迪迅生物基因工程有限公司

Various allergenic proteins are produced by house dust mites (HDM). However, the allergenicity and clinical implications of these allergens are unknown.

In patients allergic to mites IgE antibody test with a natural allergen extract does not reveal the nature of the sensitizing molecules. Polisensitization to House Dust Mites (HDM) and Storage Mites (SM) is frequently observed. The aim of this study is to define whether determination of molecular patterns of sensitization is useful for optimizing the diagnosis of these patients.

Background: Component-resolved diagnostics using specific IgE to 2 S albumins has shown to be a valuable new option in diagnostic procedure. Ana o 3 is a 2 S albumin from cashew. The aim of this study was to investigate the role of Ana o 3-specific serum IgE in the diagnosis of cashew allergy and to identify cut-off levels to replace oral food challenges. Moreover, the value of additional determination of total IgE has been investigated.

Today, in vivo allergy diagnosis and allergen-specific immunotherapy (AIT) are still based on allergen extracts obtained from natural allergen sources. Several studies analyzing the composition of natural allergen extracts have shown severe problems regarding their quality such as the presence of undefined nonallergenic materials, contaminants as well as high variabilities regarding contents and biological activity of individual allergens. Despite the increasing availability of sophisticated analytical technologies, these problems cannot be overcome because they are inherent to allergen sources and methods of extract production.

Food allergy is a serious disease worldwide; it can significantly lower the standard of living of affected individuals and may be life-threatening. In particular, hypersensitivity to seafood has been increasing in recent years owing to rising consumption. The mucosal immune system plays a critical role in the onset of seafood allergy and other allergic diseases. Recently, experimental and clinical evidence has shown that probiotics significantly modulate immune responses and thus suppress allergic reactions.

RATIONALE: Mites are one the most important sources of allergens associated with allergic diseases. Although immunotherapy is the only form of treatment that can alleviate these conditions, allergenic extracts used for such purposes are not well defined.

RATIONALE: Peanut is an important food allergen because of its clinical severity and increasing prevalence, especially in some western countries. However few data came from China.METHODS: A careful history; skin prick test for peanut; specific IgE for peanut (ImmunoCAP); peanut challenge test, SDS-PAGE electrophoresis for peanut extract, immunoblot.

House dust mites (HDMs) represent one of the most important inducers of respiratory allergies worldwide.

Background: Bet v 1 and homologous proteins represent major allergens for almost 95% of patients allergic to tree pollen and approximately 70% of those allergic to fruits and vegetables. As yet, no continuous (sequential) IgE epitopes have been determined for Bet v 1, and evidence has accumulated that Bet v 1 IgE epitopes belong to the conformational (discontinuous) type. Objective: A panel of 85 mouse monoclonal anti-Bet v 1 antibodies was raised as a tool with which to study the interaction of human IgE antibodies with Bet v 1. Methods: The epitopes of selected monoclonal antibodies (mAbs) were characterized by mapping with synthetic overlapping peptides and by cross-competition experiments.

Prior studies indicate that hazelnut component testing (HCT) may serve as a better indicator for clinical reactivity than skin prick (SPT) and hazelnut-specific IgE testing (hIgE) in patients with birch pollen allergy. The efficacy of HCT in determining clinical reactivity represents a potential gap in clinical management.

Tree nuts allergy is one of the most common food allergies. In our study we evaluate the sensitization profile in our population.

Food allergy to hazelnut occurs both with and without concomitant pollen allergy.

Soy allergy is one of the most common food allergies. The presence of IgE specific to soy component storage proteins Gly m4, m5, and m6 has been indicated as a means to improve the specific identification of systemic and local soy allergic reactions.

Incidence of food allergy is increasing, including diseases with apparently unique pathophysiologic pathways such as eosinophilic esophagitis (EoE), milk anaphylaxis and galactose-alpha-1,3-galactose (alpha-gal) allergy. The role of IgE in these diseases has been well studied, though the contribution of other antibody subtypes, including IgG4, is less clear.

Previous studies have demonstrated insufficient sensitivity of commercially available celeriac extract reagents in the diagnosis of celeriac allergy.

Sensitization to wheat flour plays an important role in the development and diagnosis of baker's asthma.

Detection of IgE to recombinant Hymenoptera venom allergens has been suggested to improve the diagnostic precision in Hymenoptera venom allergy. However, the frequency of sensitization to the only available recombinant honeybee venom (HBV) allergen, rApi m 1, in patients with HBV allergy is limited, suggesting that additional HBV allergens might be of relevance.

Food sIgG and sIgG4 are highly individually versatile. We put a hypothesis that one of the responsible factors is the presence of gastrointestinal inflammatory diseases. The objectives were: 1. An analysis of wheat and rice sIgG and sIgG4 in healthy children, children with IgE-mediated wheat allergy (WA), coeliac disease (CD) and Helicobacter pylori infection (HP). 2. Usability of wheat sIgG and sIgG4 in the WA diagnostics.

IgG4-related disease (IgG4-RD) is a systemic disorder, which is characterized by elevated serum IgG4 concentrations and infiltration of abundant IgG4 positive plasma cells in the multiple lesions. It has been reported the lesions of IgG4-RD present Th2 dominant immunity. And the patient often shows allergic history and/or symptoms. The aim of this study is to investigate allergic involvement in patients with IgG4-RD.

Allergen-specific IgG4 (sIgG4) antibodies are often associated with tolerance, but sIgG4 antibodies to causally relevant foods have been reported recently in adults with eosinophilic esophagitis (EoE). Prevalence and levels of food sIgG4 are not well established in the general pediatric population.