① 全基因组协会（GWA）的研究已经明确与哮喘发生相关的基因，但是没有研究哮喘缓解；② 临床缓解（ClinR）的定义是，最近一年中没有哮喘治疗和喘息，最近3年内无哮喘发作，完全缓解（ComR）在临床缓解的条件基础上，还必须保持正常肺功能和无支气管高反应性（BHR）；③ 评估了2个独立群体(总n = 456)中25个顶端单核苷酸多态性(SNPs)的复制情况，然后对肺组织和上皮细胞中4个复制的SNP进行定量位点表达(eQTL)分析；④ 本研究确定了3个与完全哮喘缓解相关的SNP，其中2个SNP与FRS2、CCT、IL1RL1、IL18R1和IL13有生物学相关性；⑤ 本研究三个相关SNP分别为rs7240102、rs6581895和rs1420101。
Clinical & Experimental Allergy
Novel genes and insights in complete asthma remission: A genome-wide association study on clinical and complete asthma remission
Background: Asthma is a chronic respiratory disease without a cure,although there exists spontaneous remission.Genome-wide association (GWA) studies have pinpointed genes associated with asthma development, but did not investigate asthma remission.
Methods: Clinical remission (ClinR) was defined by the absence of asthma treatment and wheezing in the last year and asthma attacks in the last 3 years and complete remission (ComR) similarly but additionally with normal lung function and absence of bronchial hyperres -ponsiveness (BHR). A GWA study on both ClinR and ComR was performed in 790 asthmatics with initial doctor diagnosis of asthma and BHR and long-term follow-up. We assessed replication of the 25 top single nucleotide polymorphisms (SNPs) in 2 independent cohorts (total n = 456), followed by expression quantitative loci (eQTL) analyses of the 4 replicated SNPs in lung tissue and epithelium.
Results: Of the 790 asthmatics, 178 (23%) had ClinR and 55 ComR (7%) after median follow-up of 15.5 (range 3.3-47.8) years. In ClinR, 1 of the 25 SNPs, rs2740102,replicated in a meta-analysis of the replication cohorts, which was an eQTL for POLI in lung tissue. In ComR, 3 SNPs replicated in a meta-analysis of the replication cohorts. The top-hit, rs6581895, almost reached genome-wide significance (P-value 4.68 9 107) and was an eQTL for FRS2 and CCT in lung tissue. Rs1420101 was a cis-eQTL in lung tissue for IL1RL1 and IL18R1 and a trans-eQTL for IL13.
Conclusions: By defining a strict remission phenotype, we identified 3 SNPs to be associated with complete asthma remission, where 2 SNPs have plausible biological relevance in FRS2, CCT, IL1RL1, IL18R1 and IL13.
J. M. Vonk
Department of Epidemiology, University Medical Center Groningen, University of Groningen,Groningen, The Netherlands
We describe the architecture of the evolution of IgE responses to multiple allergen components throughout childhood, which may facilitate development of better diagnostic and prognostic biomarkers for allergic diseases.
J. M. Vonk, M. A. E. Nieuwenhuis, F. N. Dijk, A. Boudier, V. Siroux, E. Bouzigon, N. Probst-Hensch, M. Imboden, D. Keidel, D. Sin, Y. Bosse, K. Hao, M. van den Berge, A. Faiz, G. H. Koppelman, D. S. Postma