①屋尘螨(HDMs)是世界范围内最重要的呼吸道过敏诱因之一。②我们试图研究过敏性哮喘儿童中HDM过敏原的IgE和IgG反应谱，并将其与非哮喘性特应性儿童进行比较。③用一系列户尘螨过敏原(nDer p 1, rDer p 2, rDer p 5, rDer p 7, rDer p 10, rDer p 21, and rDer p 23)通过过敏原微阵列技术对来自明确诊断的户尘螨过敏哮喘儿童 (n = 105),非哮喘儿童(n = 53),非过敏且非哮喘儿童 (n = 53)的血清 进行了IgE结合分析。④户尘螨过敏的哮喘患儿对每种户尘螨过敏原的IgE反应频率更高，识别出的过敏原多于对户尘螨过敏的非哮喘患儿。此外，某些户尘螨过敏原的IgE水平（nDer p 1, P = .002; rDer p 2, P = .007; rDer p 5, P = .031; and rDer p 23, P < .001 rDer p23、p < 0.001）哮喘患儿显著高于非哮喘患儿。相比之下，哮喘患儿对户尘螨过敏原有IgG反应活性的人数低于非哮喘患儿，但过敏原特异性IgG水平相当。⑤对户尘螨过敏原的IgE和IgG反应谱，以及对某些过敏原组分的IgE水平，在由户尘螨过敏引起哮喘和非哮喘儿童之间有很大差异。事实上，哮喘患儿的特点是IgE结合过敏原组份的数量增加，且特异性IgE水平升高。
Different IgE recognition of mite allergen components in asthmatic and nonasthmatic children
House dust mites (HDMs) represent one of the most important inducers of respiratory allergies worldwide.
We sought to investigate the IgE and IgG reactivity profiles to a comprehensive panel of HDM allergens in children with allergic asthma and to compare them with those of nonasthmatic atopic children.
Sera from clinically well-characterized asthmatic children with HDM allergy (n = 105), nonasthmatic children (n = 53), and nonatopic nonasthmatic children (n = 53) were analyzed for IgE and IgG reactivity to a panel of 7 HDM allergens (nDer p 1, rDer p 2, rDer p 5, rDer p 7, rDer p 10, rDer p 21, and rDer p 23) by means of allergen microarray technology.
Asthmatic children with HDM allergy more frequently showed an IgE response to each of the HDM allergens and recognized more allergens than nonasthmatic children with HDM allergy. Furthermore, IgE levels to certain HDM allergens (nDer p 1, P = .002; rDer p 2, P = .007; rDer p 5, P = .031; and rDer p 23, P < .001) were significantly higher in asthmatic children than in children without asthma. By contrast, fewer asthmatic children showed IgG reactivity to HDM allergens than nonasthmatic children, but allergen-specific IgG levels were comparable.
The IgE and IgG reactivity profiles to HDM allergens, as well as IgE levels to certain allergen components, differed considerably between children with and without asthmatic symptoms caused by HDM allergy. In fact, asthmatic children were characterized by an expanded IgE repertoire regarding the numbers of recognized allergen components and by increased specific IgE levels.
Yvonne Resch, MSc, Sven Michel, MSc, Michael Kabesch, MD, Christian Lupinek, MD, Rudolf Valenta, MD, Susanne Vrtala, PhD